Shared Resources


Transgenic and Chimeric Mouse Facility

Director: Nancy Cooke, M.D. and Stephen Liebhaber, M.D.

The Transgenic and Chimeric Mouse Facility(TCMF) was established in 1989. The Facility provides a single centralized laboratory to generate pathogen-free mice carrying transgenes or targeted structural and functional alterations of endogenous genes of specific interest to individual investigators. The centralization of these technically demanding procedures results in enhanced efficiency and reduced costs for each user’s project. The generation of mouse models facilitates collaborations and interactions among cancer researchers. To reduce cage costs to users and to provide protection against loss of lines, the Core also offers embryo and sperm cryopreservation, long-term cryo storage for embryos and sperm, and in vitro fertilization and for line revival.

Staff

Nancy E. Cooke, MD, Director

Stephen A. Leibhaber, MD, Co-Director

Contact

Nancy E. Cooke
Division of Endocrinology, Diabetes, & Metabolism
415 Curie Blvd., 547A CRB
Philadelphia, PA 19104-6149
Phone: (215) 898-4425
Fax: (215) 573-5809

Stephen Liebhaber
560A Clinical Research Building
415 Curie Boulevard
Philadelphia, PA 19104-6145
Office: 215-898-7834
Fax: 215-573-5157
Email: liebhabe@mail.med.upenn.edu

Locations

TCMF Core Laboratory is located in the basement of the Clinical Research Building within a microbiological barrier environment. The microinjection laboratory occupies rooms 38 and 40 (239 and 248 square feet, respectively) that were renovated and physically joined in 2004.

TCMF Core mouse cage facility is located in the adjacent room 34.

TCMF Core Cryopreservation Storage Facility, is located in the Anatomy Chemistry Suite B22.

Fees

A. Mouse Transgenics: Introduction of DNA constructs into fertilized oocytes.

$1,250 / DNA construct for ACC members.

$2,500 / DNA construct for other Penn Investigators.

$4,250 / DNA construct (full cost) for investigators outside of Penn, (for example faculty at neighboring institutions such as Temple University and Princeton University) provided on 'time-available' basis.

B. Mouse Chimerics: Introduction of embryonic stem cells into blastocysts.

$1,250 / cell line for ACC members. ($425 to test non-recombinant ES cell line for its ability to contribute to chimerism and/or germline.)

$2,500 / DNA cell line for other Penn Investigators.

$4,250 / DNA cell line (full cost) for investigators outside of Penn, provided on
'time-available' basis.

C. Transient Transgenics: Direct provision of founder embryos.

$1,000 / DNA construct for ACC members.

$2,000 / DNA construct for other Penn Investigators.

$3,400 / DNA construct (full cost) for investigators outside of Penn, provided on 'time-available' basis.

D. Embryo Cryopreservation: $625 ($500 for ACC members) if investigators to do the matings and $ 1,250 / line frozen ($1,000 for ACC members) if the Core is requested to do the matings.

E. Oocyte Harvest with/without transfer to oviduct: Price based upon the fraction of time involved, that is, 0.4 ($500) or 0.3 ($375) respectively, of the mouse transgenic service in A.

F. Embryo Re-derivation: $625 / line ($500 for ACC members) - full service (embryo derivation and re-implantation). Investigator supplies embryos and bears the cost of quarantine housing.

G. Sperm Cryopreservation: $625 / service ($500 for ACC members).

H. In vitro Fertilization: $625 / service ($500 for ACC members).

Equipment

  • Four fully equipped microinjection stations suitable for DNA injection of embryos and ES injection of blastocysts. One of these stations is also equipped for Intracytoplasmic Sperm Injection (ICSI) with a piezo drill.
  • All microinjection stations are positioned on vibration-stabilization platforms.
  • Two stereomicroscopes for dissections.
  • Pipette puller, forge, and grinder
  • Water bath
  • pH meter
  • PowerMAC
  • Two Eppendorf microfuges
  • Refrigerator and freezer
  • Liquid N2 dewar
  • Nikon Coolpix camera and color monitor
  • TV monitor
  • Biocool III apparatus
  • CO2 incubator
  • Dry sterilizer

For more information, you can also visit the Core's website: http://www.med.upenn.edu/genetics/tcmf/